The resulting 3D artificial tissues were examined and characterized by normal phase, oblique dark field, SEM and immunofluorescence microscopy. The reported complex tissues generated a good deal of interest among the participants of the meeting and resulted in numerous new contacts for future projects. The meeting also provided the TCIC with critical new insights into the state of the art in stem cell sciences and gave us a good preview of future directions in the field. These new perspectives served to confirm that our work is on track to contribute to understanding the biology of these important cells in the 3D artificial tissue context. In discussions with several other labs and companies at the meeting, new collaborative efforts to explore 3D culture of other cell types has been planned for the future. The World Stem Cell Summit is a premier gathering of Stem Cell biologists held annually since 2007 with around 1500 participants each year. Next year’s event will be held in Pasadena California.
TCIC presents at the 2010 World Stem Cell Summit
The 2010 World Stem Cell Summit was held in Detroit at the Marriott Center from October 2-5. This year the TCIC joined this premier meeting with the presentation of a poster entitled “Characterization of Human Embryonic Stem Cell-Derived 3D Artificial Tissue Structures Produced with Natural ECM Scaffolding Materials”. Director, Tim Lyden was joined in Detroit by TCIC student researcher, Tim Morris who helped present the poster in three days of presentation sessions. This poster reported on studies undertaken over the past 3 years and also involved student researcher Travis Cordie, who is now employed at WiCell, the NIH National Stem Cell Bank in Madison WI. These studies have explored the application of 3D culture technologies developed at the TCIC for the culture and development of human embryonic stem cells in vitro. 
The resulting 3D artificial tissues were examined and characterized by normal phase, oblique dark field, SEM and immunofluorescence microscopy. The reported complex tissues generated a good deal of interest among the participants of the meeting and resulted in numerous new contacts for future projects. The meeting also provided the TCIC with critical new insights into the state of the art in stem cell sciences and gave us a good preview of future directions in the field. These new perspectives served to confirm that our work is on track to contribute to understanding the biology of these important cells in the 3D artificial tissue context. In discussions with several other labs and companies at the meeting, new collaborative efforts to explore 3D culture of other cell types has been planned for the future. The World Stem Cell Summit is a premier gathering of Stem Cell biologists held annually since 2007 with around 1500 participants each year. Next year’s event will be held in Pasadena California.
The resulting 3D artificial tissues were examined and characterized by normal phase, oblique dark field, SEM and immunofluorescence microscopy. The reported complex tissues generated a good deal of interest among the participants of the meeting and resulted in numerous new contacts for future projects. The meeting also provided the TCIC with critical new insights into the state of the art in stem cell sciences and gave us a good preview of future directions in the field. These new perspectives served to confirm that our work is on track to contribute to understanding the biology of these important cells in the 3D artificial tissue context. In discussions with several other labs and companies at the meeting, new collaborative efforts to explore 3D culture of other cell types has been planned for the future. The World Stem Cell Summit is a premier gathering of Stem Cell biologists held annually since 2007 with around 1500 participants each year. Next year’s event will be held in Pasadena California.
TCIC Director presents at UW-Whitewater Biology Colloquium
On October 29th, the TCIC Director, Dr. Lyden presented an invited seminar entitled “3D Cell Culture Modeling of Developmental Processes: Normal and Pathologic“ in the Biology Department Fall 2010 Colloquium at the University of Wisconsin-Whitewater. Hosted by Dr. Eric Brown, Assistant Professor of NanoBiotechnology, the talk focused on the application of 3D cell culture in modeling cell lines, primary developmental tissues and primary tumor tissues from human patients in translational medicine projects currently ongoing in the lab. The talk was attended by some 70-80 students and faculty from the department. The talk was well received, generating excellent questions and discussions over lunch following the presentation.
TCIC Crew at the 2010 Midwest Medical Design and Manufacturing Tradeshow
On October 10th, four students from the TCIC, Tim Morris, Kevin Rixmann, Ashley Graef and Reid Kuen joined the Director, Dr. Lyden at the Midwest Medical Design and Manufacturing Tradeshow held at the Minneapolis Convention Center. This show is one of the largest in the Midwest and will bring together more than 300 exhibitors and some 3000 visitors to display the regions biomedical devices industry and related partners from across the nation. The TCIC group helped man a booth for the Momentum West organization based in Eau Claire. Momentum West is the regional economic development organization in western Wisconsin. The booth featured materials from across this region of Wisconsin and included information on the UWRF Tissue and Cellular Innovation Center. In addition to covering the booth, the TCIC crew also explored the show and made many new contacts as well as gained insights into the current and future state of the art in biomedical device technology.
Wisconsin Science and Technology Symposium, July 22-23, 2010
On July 22-23, the 3rd Annual Wisconsin Science and Technology Symposium (WSTS 2010) was held at the UW-Green Bay. This annual gathering brings together scientists, industrial partners and politicians from across the state to share research from the comprehensive campuses and technology industries of the region. Again this year, two major laboratories from the Biology Department participated in this premier statewide meeting. Both the UWRF Tissue and Cellular Innovation Center and the UWRF Zebra Fish Laboratory presented a combined total of 8 posters and two oral presentations. This total, which amounted to approximately 31% of the posters at the meeting exceeded any other campus represented and was very well received with a great deal of interest being shown by other participants toward the biological research done here at UWRF. Drs. Lyden and Huang, the respective directors of the labs, were again joined this year by undergraduate researchers who presented their work in the all day poster sessions. Meanwhile Dr Lyden presented a podium talk entitled “In-vitro 3D Artificial Tumor Microenvironments as Potential Models of Clinical Disease“ which focused on the TCIC human tumor 3D modeling project being conducted in collaboration with Marshfield Clinic and the Rivers Cancer Center. Dr. Huang also presented a podium talk entitled “Zebrafish Make a Splash in Drug Discovery“ which highlighted work done in his lab in collaboration with researchers at the UW-LaCrosse Mycology Discovery Lab. UWRF student presenters were: Tim Morris, Kevin Rixmann and Reid Kuen from the TCIC as well as David Mankowski and Johnathan Emahiser from the Zebra Fish Lab. UWRF Posters presented were entitled:
C6, a Potential Drug for Heart Failure by Suppressing Inflammation.
Johnathan Emahiser and Cheng-Chen Huang, UW-River Falls
Screening Synthetic Chemicals and Purified Compounds from Natural Sources for Attenuative Action against AA-induced Heart Failure in Zebrafish.
David A Mankowski and Cheng-Chen Huang, UW-River Falls
Modeling complex cervical carcinoma cell-derived structures in 3D “artificial tissue”cultures.
Brittany Lee and Timothy Lyden, UW-River Falls
The isolation and 3D culture of putative fetal and adult cardiac stem cell populations from avian, bovine and porcine heart samples.
Timothy Lyden, Mike Martin, Victor Piazza, John Magnuson and Travis Cordie, UW-River Falls and Spring Point Project LLC
Modeling and characterization of primary and cell-line derived artificial breast cancer tissues produced using 3D culture methods.
Kevin Rixmann and Timothy Lyden, UW-River Falls; Ray Haselby, Marshfield Clinic; Peter Dahlberg, Rivers Cancer Center
Longterm 3-D cultures of HEK-293 cells demonstrate clear evidence of unique
kidney tissue-like differentiation.
Miyuki Bessho, Timothy Lyden, UW-River Falls
Seeking to generate “induced pluripotent stem” (IPS) cells from the cervical cancer cell line, HeLa.
Timothy A Morris Jr., Samuel Lifton, Reid Kuen, Timothy Lyden, UW-River Falls
Modeling human cancer with complex 3D cultures using rudimentary tissue
engineering methods.
Timothy Lyden, UW-River Falls; Ray Haselby, Marshfield Clinic; Peter Dahlberg, Rivers Cancer Center; Michael Pickart, UW-Stout
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